A photoswitchable GPCR-based opsin for presynaptic inhibition.
Copits BA, Gowrishankar R, O'Neill PR, Li JN, Girven KS, Yoo JJ, Meshik X, Parker KE, Spangler SM, Elerding AJ, Brown BJ, Shirley SE, Ma KKL, Vasquez AM, Stander MC, Kalyanaraman V, Vogt SK, Samineni VK, Patriarchi T, Tian L, Gautam N, Sunahara RK, Gereau RW 4th, Bruchas MR
Neuron. 2021 Jun 2;109(11):1791-1809.e11. doi: 10.1016/j.neuron.2021.04.026. Epub 2021 May 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139502 | pcDNA3-PPO-Venus | Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades. |
| 139503 | pcDNA3-PPO-mScarlet | Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades. |
| 139504 | pAAV-CAG-DIO-PPO-Venus | AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades. |
| 139505 | pAAV-Ef1a-DIO-PPO-Venus | AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades. |
| 140097 | pcDNA3-mScarlet-gamma9 | Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation |