A photoswitchable GPCR-based opsin for presynaptic inhibition.

Copits BA, Gowrishankar R, O'Neill PR, Li JN, Girven KS, Yoo JJ, Meshik X, Parker KE, Spangler SM, Elerding AJ, Brown BJ, Shirley SE, Ma KKL, Vasquez AM, Stander MC, Kalyanaraman V, Vogt SK, Samineni VK, Patriarchi T, Tian L, Gautam N, Sunahara RK, Gereau RW 4th, Bruchas MR
Neuron. 2021 Jun 2;109(11):1791-1809.e11. doi: 10.1016/j.neuron.2021.04.026. Epub 2021 May 11. PubMed Article

Plasmids from Article

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ID	Plasmid	Purpose
140097	pcDNA3-mScarlet-gamma9	Expresses an mScarlet-tagged gamma9 subunit for visualizing G beta-gamma translocation
139505	pAAV-Ef1a-DIO-PPO-Venus	AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the Ef1a promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
139504	pAAV-CAG-DIO-PPO-Venus	AAV vector for Cre-dependent expression of Venus-tagged parapinopsin driven by the CAG promoter. Used for photoswitchable control of inhibitory GPCR signaling cascades.
139503	pcDNA3-PPO-mScarlet	Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.
139502	pcDNA3-PPO-Venus	Expresses Venus-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.