Precise and Programmable Detection of Mutations Using Ultraspecific Riboregulators.
Hong F, Ma D, Wu K, Mina LA, Luiten RC, Liu Y, Yan H, Green AA
Cell. 2020 Feb 25. pii: S0092-8674(20)30155-0. doi: 10.1016/j.cell.2020.02.011.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139463 | p_SNIPR_sensor_GFP | T7 RNAP-driven expression of SNIPR RNA with a GFP-ASV reporter |
| 139464 | p_SNIPR_sensor_LacZ | T7 RNAP-driven expression of SNIPR RNA with a LacZ reporter |
| 139465 | p_SNIPR_target_WT | T7 RNAP-driven expression of the cognate SNIPR trigger RNA that is complementary to the SNIPR RNA sensor |
| 139466 | p_SNIPR_target_mut_36 | T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 36 |
| 139467 | p_SNIPR_target_42_mut_G | T7 RNAP-driven expression of WT trigger RNA with mutation at 42 |
| 139468 | p_SNIPR_target_GUmut33AtoG | T7 RNAP-driven expression of a non-cognate SNIPR trigger RNA with a mutation at position 33 |