Broadening the spectrum of fluorescent protein tools for use in the encapsulated human fungal pathogen Cryptococcus neoformans.
Spencer GWK, Chua SMH, Erpf PE, Wizrah MSI, Dyba TG, Condon ND, Fraser JA
Fungal Genet Biol. 2020 May;138:103365. doi: 10.1016/j.fgb.2020.103365. Epub 2020 Mar 4.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139410 | pGWKS2 | Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised GFP S65T marker. |
| 139411 | pGWKS4 | Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mCherry marker. |
| 139412 | pGWKS5 | Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker. |
| 139413 | pGWKS6 | Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mMaroon1 marker. |
| 139414 | pGWKS7 | Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker. |
| 139415 | pGWKS8 | Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTFP1 marker. |
| 139416 | pGWKS9 | Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mTurquoise2 marker. |
| 139417 | pGWKS10 | Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker. |
| 139418 | pGWKS11 | Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mNeonGreen marker. |
| 139419 | pGWKS12 | Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Superfolder GFP marker. |
| 139420 | pGWKS17 | Fluoroescent marker for use in Cryptococcus neoformans. Contains a non-codon optimised mCherry marker. |
| 139421 | pPEE42 | Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the Citrine ORF and a flexible linker at the 5' end. |
| 139422 | pPEE43 | Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mMaroon1 ORF and a flexible linker at the 5' end. |
| 139423 | pPEE44 | Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTFP1 ORF and a flexible linker at the 5' end. |
| 139424 | pPEE45 | Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mTurquoise2 ORF and a flexible linker at the 5' end. |
| 139425 | pPEE46 | Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end. |
| 139426 | pPEE47 | Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end. |