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Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics.

Uda Y, Miura H, Goto Y, Yamamoto K, Mii Y, Kondo Y, Takada S, Aoki K
ACS Chem Biol. 2020 Nov 20;15(11):2896-2906. doi: 10.1021/acschembio.0c00477. Epub 2020 Nov 9. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
139477pCAGGS-synPCB2.0Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
139478pCAGGS-synPCB2.1Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
139479pCAGGS-synPCB2.2Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
139480pCSIIhyg-rtTAExpresses rtTA, Tet-ON reverse tetracycline-controlled trans-activator in mammalian cells.
139481pT2Apuro-TRE-synPCB2.0Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.
139482pCSIIneo-TRE-hPhyB621- mCherry-HRasCTExpresses PhyB621-mCherry-HRasCT in mammalian cells.
139483pCSIIbleo-PIF3-mEGFPExpresses PIF3-mEGFP in mammalian cells.

Antibodies from Article