Genetic circuit design automation for the gut resident species Bacteroides thetaiotaomicron.
Taketani M, Zhang J, Zhang S, Triassi AJ, Huang YJ, Griffith LG, Voigt CA
Nat Biotechnol. 2020 Aug;38(8):962-969. doi: 10.1038/s41587-020-0468-5. Epub 2020 Mar 30.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139382 | pMT423 | Constitutively expressed dCas9, LacI and TetR, pNBU2 backbone, AmpR, TetR |
| 139383 | pMT405 | LacZ, pNBU1 backbone with BsaI sites, AmpR, ErmR |
| 139384 | pMT406 | RPU standard, pNBU1 backbone with BsaI sites, AmpR, ErmR |
| 139385 | pMT444 | IPTG inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR |
| 139386 | pMT445 | aTC inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR |
| 139387 | pMT447 | Bile Acid Inducible Nanoluc expression, pNBU1 backbone with BsaI sites, AmpR, ErmR |
| 139388 | pMT448 | Bile Acid inducible sgRNA-1 with PM1 driving Nanoluc expression (NOT Gate 1), pNBU1 backbone, AmpR, ErmR |
| 139389 | pMT449 | Bile Acid inducible sgRNA-2 with PM2 driving Nanoluc expression (NOT Gate 2), pNBU1 backbone, AmpR, ErmR |
| 139390 | pMT450 | Bile Acid inducible sgRNA-3 with PM3 driving Nanoluc expression (NOT Gate 3), pNBU1 backbone, AmpR, ErmR |
| 139391 | pMT451 | Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmR |
| 139392 | pMT455 | aTC and Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOR Gate), AmpR, ErmR |
| 139393 | pMT462 | XOR circuit (A000 and P000 design) with aTC and Bile Acid as inputs, AmpR, ErmR |
| 139394 | pMT468 | Nanoluc placed on Output 2 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs, AmpR, ErmR |
| 139395 | pMT469 | Nanoluc placed on Output 1 of the 2in3out circuit (A001 and P002 design) with aTC and Bile Acid as inputs, AmpR, ErmR |
| 139397 | pMT492 | Bile Acid inducible sgRNA-5 with PM5 driving Nanoluc expression (NOT Gate 5), pNBU1 backbone, AmpR, ErmR |
| 139398 | pMT493 | Bile Acid inducible sgRNA-6 with PM6 driving Nanoluc expression (NOT Gate 6), pNBU1 backbone, AmpR, ErmR |
| 139399 | pMT494 | Bile Acid inducible sgRNA-7 with PM7 driving Nanoluc expression (NOT Gate 7), pNBU1 backbone, AmpR, ErmR |