Detection of Marker-Free Precision Genome Editing and Genetic Variation through the Capture of Genomic Signatures.

Billon P, Nambiar TS, Hayward SB, Zafra MP, Schatoff EM, Oshima K, Dunbar A, Breinig M, Park YC, Ryu HS, Tschaharganeh DF, Levine RL, Baer R, Ferrando A, Dow LE, Ciccia A
Cell Rep. 2020 Mar 10;30(10):3280-3295.e6. doi: 10.1016/j.celrep.2020.02.068. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
139321	sgRNA BRCA2 V572I	Plasmid expressing a sgRNA to introduce BRCA2 V572I using base editing
139322	sgRNA BRCA2 V2102I	Plasmid expressing a sgRNA to introduce BRCA2 V2102I using base editing
139323	sgRNA BRCA2 E2772K	Plasmid expressing a sgRNA to introduce BRCA2 E2772K using base editing
139324	sgRNA BRCA2 R2842C	Plasmid expressing a sgRNA to introduce BRCA2 R2842C using base editing
139325	sgRNA BRCA2 S3070F	Plasmid expressing a sgRNA to introduce BRCA2 S3070F using base editing
139326	sgRNA BRCA1 C64Y	Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing
139327	sgRNA BRCA1 E638K	Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing
139328	sgRNA BRCA1 T922I	Plasmid expressing a sgRNA to introduce BRCA1 T922I using base editing
139329	sgRNA BRCA1 S1363L	Plasmid expressing a sgRNA to introduce BRCA1 S1363L using base editing
139330	sgRNA BRCA1 E1754K	Plasmid expressing a sgRNA to introduce BRCA1 E1754K using base editing
139331	sgRNA BRCA1 Q1779Stop	Plasmid expressing a sgRNA to introduce BRCA1 Q1779Stop using base editing
139332	sgRNA FANCD2 Q223Stop	Plasmid expressing a sgRNA to introduce FANCD2 Q223Stop using base editing
139333	DTECT - Plasmid for Standard Curve	This plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECT
139511	sgRNA BRCA2 R2896C	Plasmid expressing a sgRNA to introduce BRCA2 R2896C using base editing