Probing the subcellular distribution of phosphatidylinositol reveals a surprising lack at the plasma membrane.
Zewe JP, Miller AM, Sangappa S, Wills RC, Goulden BD, Hammond GRV
J Cell Biol. 2020 Mar 2;219(3). pii: 133808. doi: 10.1083/jcb.201906127.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139306 | iRFP-N1-PI-PLCC100-FRB | C-terminal half of split PI-PLC |
| 139307 | TagBFP2-C1-FKB-PI-PLCN187 | N-terminal half of split PI-PLC |
| 139308 | pTagBFP2-C1-lynN11-FKBP-PI-PLCN187 | N-terminal half of split PI-PLC targeted to plasma membrane |
| 139309 | pTagBFP2-C1-AKAP1N31-FKBP-PI-PLCN187 | N-terminal half of split PI-PLC targeted to mitochondria |
| 139310 | mCherry-FKBP-PI4KB | Recruitable PI4KB |
| 139311 | mCherry-FKBP-PI4KAC1001 | Recruitable PI4KA catalytic fragment |
| 139312 | mCherry-FKBP-PI4KAC1001(D1957A) | Recruitable PI4KA kinase-dead catalytic fragment |
| 139313 | piRFP-FRB-Giantin | Golgi-resident FRB for chemically induced dimerization |
| 139314 | pmCherry-C1ab-Prkd1 | Diacylglycerol biosensor |
| 139315 | Akap1(31)-FRB-iRFP | mitochondrial-resident FRB for chemically induced dimerization |
| 139316 | piRFP-FRB-PMP-C-10 | peroxisome-resident FRB for chemically induced dimerization |
| 139317 | iRFP-FRB-ER | endoplasmic reticulum-resident FRB for chemically induced dimerization |