Neal Devaraj Lab: Methylxanthine Synthesis in Human Cells
Unpublished
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 139159 | pSBBi-RP PcCS | SB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana caffeine synthase (PcCS); the other side contains RFP and puromycin resistance genes. |
| 139160 | pSBBi-RP GST-PcCS | SB-transposon with constitutive bi-directional promoter. One side contains N-GST Paullinia cupana caffeine synthase; the other side contains RFP and puromycin resistance genes. |
| 139161 | pSBBi-RP TCS1 | SB-transposon with constitutive bi-directional promoter. One side contains tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes. |
| 139162 | pSBBi-RP CCS1 | SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1); the other side contains RFP and puromycin resistance genes. |
| 139163 | pSBBi-RP CCS1(Δ304-316) | SB-transposon with constitutive bi-directional promoter. One side contains coffee caffeine synthase 1 (CCS1) CCS-CTS deletion mutant; the other side contains RFP and puromycin resistance genes. |
| 139164 | pSBBi-RP GST-CCS1 | SB-transposon with constitutive bi-directional promoter. One side contains N-GST coffee caffeine synthase 1 (GST-CCS1) fusion; the other side contains RFP and puromycin resistance genes. |
| 139165 | pSBBi-RP CaDXMT | SB-transposon with constitutive bi-directional promoter. One side contains Coffee arabica dimethylxanthine methyl transferase (CaDXMT) ; the other side contains RFP and puromycin resistance genes. |
| 139166 | pSBBi-RP GST-TCS1 | SB-transposon with constitutive bi-directional promoter. One side contains N-GST tea caffeine synthase 1 (TCS1) from Camellia sinensis; the other side contains RFP and puromycin resistance genes. |
| 139167 | pSBBi-RP CsAncCS | SB-transposon with constitutive bi-directional promoter. One side contains Camellia sinensis ancestral caffeine synthase (CsAncCS); the other side contains RFP and puromycin resistance genes. |
| 139168 | pSBBi-GB CaXMT1 | SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes. |
| 139169 | pSBBi-GB GST-CaXMT1 | SB-transposon with constitutive bi-directional promoter. One side contains N-GST Coffea arabica xanthosine methyltransferase 1 (CaXMT1); the other side contains GFP and blasticidin resistance genes. |
| 139170 | pSBBi-RP PcAncCS2 | SB-transposon with constitutive bi-directional promoter. One side contains Paullinia cupana ancestral Caffeine Synthase 2 (PcAncCS2); the other side contains RFP and puromycin resistance genes. |
| 139171 | pSBBi-GB TCS2 | SB-transposon with constitutive bi-directional promoter. One side contains tea caffeine synthase 1 (TCS2) from Camellia sinensis; the other side contains GFP and blasticidin resistance genes. |
| 139172 | pSBBi-RP CaMXMT | SB-transposon with constitutive bi-directional promoter. One side contains Coffea arabica monomethylxanthine methyltransferase (CaMXMT); the other side contains RFP and puromycin resistance genes. |
| 139176 | pSBTet(-ΔrtTA)-GB dTomato-P2A-CaMXMT | SB-transposon with inducible expression of dTomato and Coffea arabica monomethylxathine methyltransferase via P2A self-cleaving linker in a Tet-inducible pSBTRE(-ΔrtTA)-GB backbone. |
| 139184 | pSB-Bleo STAT3 | Empty backbone encoding constitutive bleomycin resistance with an inducible STAT3 promoter to allow for an insert in a Sleeping Beauty backbone. |
| 139185 | pSBBi-Pur GFP-TM | SB-transposon with constitutive bi-directional promoter. One side contains transmembrane-anchored GFP; the other side contains the puromycin resistance gene. |
| 139189 | pSB-Bleo Gal4 CCS1 | SB-transposon with constitutive bleomycin resistance and Gal4-inducible coffee caffeine synthase 1 (CCS1). |
| 139190 | pSB-Bleo Gal4 tBFP | Gal4-inducible tag blue fluorescent protein (tBFP) in pSB-Bleo Gal4 Sleeping Beauty backbone. |
| 158133 | pSBBi-Pur LaG17-synNotch-TetRVP64 | Constitutive expression of anti-GFP nanobody (LaG-17) synNotch TetRVP64 receptor in a pSBBi-Pur Sleeping Beauty backbone |
| 158135 | pSBBi-Pur LaG17-synNotch-Gal4VP64 | Constitutive expression of anti-GFP nanobody (LaG-17) synNotch Gal4VP64 receptor in a pSBBi-Pur Sleeping Beauty backbone |