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Discovering tumor-reactive T-cell receptors through single-cell sequencing of tumor-infiltrating lymphocytes

Gottschalk E, Aksoy BA, Aksoy P, Swiderska-syn M, Mart C, Macpherson L, Romeo M, Smith A, Knochelmann H, Paulos C, Timmers C, Wrangle J, Rubinstein MP, Hammerbacher J
bioRxiv 2021.11.30.470597 (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
138983pcDNA3.1(+)-MC38-TCRA-30-picky_stickyIVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38
138984pcDNA3.1(+)-MC38-TCRB-30-picky_stickyIVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38
138985pcDNA3.1(+)-MC38-TCRA-31-murderous_crowIVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38
138986pcDNA3.1(+)-MC38-TCRB-31-murderous_crowIVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38
138987pcDNA3.1(+)-MC38-TCRA-32-riot_punisherIVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38
138988pcDNA3.1(+)-MC38-TCRB-32-riot_punisherIVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38
138989pcDNA3.1(+)-MC38-TCRA-33-MC_hammerIVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38
138990pcDNA3.1(+)-MC38-TCRB-33-MC_hammerIVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38
175287pcDNA3.1(+)_Pmel-1_TCRAExpresses Pmel-1 TCRA alpha chain
175288pcDNA3.1(+)_Pmel-1_TCRBExpresses Pmel-1 TCR beta chain

Antibodies from Article