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Split-wrmScarlet and split-sfGFP: tools for faster, easier fluorescent labeling of endogenous proteins in Caenorhabditis elegans.

Goudeau J, Sharp CS, Paw J, Savy L, Leonetti MD, York AG, Updike DL, Kenyon C, Ingaramo M
Genetics. 2021 Apr 15;217(4). pii: 6126424. doi: 10.1093/genetics/iyab014. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
138966pJG100Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans
158533pJG103wrmScarlet11 x3 tandem repeats
158584pRS423-GPD-wrmScarlet1-10-CYC1 terminatorExpresses wrmScarlet1-10 in S. cerevisiae
158585p416-TEF-membrane localization signal-tagBFP-wrmScarlet11-TEF terminatorExpresses membrane localized tagBFP with wrmScarlet11 C-terminal fusion in S. cerevisiae

Antibodies from Article