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An N-end rule pathway that recognizes proline and destroys gluconeogenic enzymes.

Chen SJ, Wu X, Wadas B, Oh JH, Varshavsky A
Science. 2017 Jan 27;355(6323). pii: 355/6323/eaal3655. doi: 10.1126/science.aal3655. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
104343pCSJ95Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
104344pCSJ125Express yeast MDH2-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
159723pCSJ182Express Gid4 (YBR105C) in yeast two hybrid plasmid, pGADCg

Antibodies from Article