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Biotin-independent strains of Escherichia coli for enhanced streptavidin production.

Jeschek M, Bahls MO, Schneider V, Marliere P, Ward TR, Panke S
Metab Eng. 2017 Mar;40:33-40. doi: 10.1016/j.ymben.2016.12.013. Epub 2017 Jan 3. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
138587pCKmatBCmatBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat
138588pET30b_T7SavT7-tagged streptavidin with native C-terminus controlled by PT7 promoter; KanR, pBR322 ori

Antibodies from Article