Efficient, continuous mutagenesis in human cells using a pseudo-random DNA editor.
Chen H, Liu S, Padula S, Lesman D, Griswold K, Lin A, Zhao T, Marshall JL, Chen F
Nat Biotechnol. 2019 Dec 16. pii: 10.1038/s41587-019-0331-8. doi: 10.1038/s41587-019-0331-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138586 | pUC19 - T7 pro - IRES - EGFP | Express EGFP with an upstream T7 promoter in pUC19 backbone |
| 138610 | pcDNA3.1(+) - Hyperactive AID - T7 RNA Polymerase -UGI - T2A - tdTamato | Express TRACE editor together with a tdTomato reporter in pcDNA3.1(+) backbone |
| 138615 | pLX - T7pro - EBFP | Express EBFP with an upstream T7 promoter in a lentiviral backbone |