G-tract RNA removes Polycomb repressive complex 2 from genes.
Beltran M, Tavares M, Justin N, Khandelwal G, Ambrose J, Foster BM, Worlock KB, Tvardovskiy A, Kunzelmann S, Herrero J, Bartke T, Gamblin SJ, Wilson JR, Jenner RG
Nat Struct Mol Biol. 2019 Oct;26(10):899-909. doi: 10.1038/s41594-019-0293-z. Epub 2019 Sep 23.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138524 | pLKO.1-puro U6 sgRNA BfuAI stuffer G-tract | Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt PRC2-binding G-tract repeat RNA sequence. |
| 138525 | pLKO.1-puro U6 sgRNA BfuAI stuffer A-tract | Expresses a guide RNA of choice cloned into the BfuI site extended at the 3' end to incorporate a 220-nt control A-tract repeat RNA sequence. |