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Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity.

Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR
Nat Biotechnol. 2020 Jul;38(7):883-891. doi: 10.1038/s41587-020-0453-z. Epub 2020 Mar 16. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
138489ABE8eA-to-G base editor
138490ABE8e-dimerA-to-G base editor
138491NG-ABE8eA-to-G base editor
138492CP1028-ABE8eA-to-G base editor
138493CP1041-ABE8eA-to-G base editor
138494ABE8e(TadA-8e V82G)A-to-G base editor
138495ABE8e(TadA-8e V106W)A-to-G base editor
138496ABE8e(TadA-8e K20A R21A)A-to-G base editor
138497NG-ABE8-dimerA-to-G base editor
138498CP1028-ABE8e-dimerA-to-G base editor
138499CP1041-ABE8e-dimerA-to-G base editor
138500SaABE8eA-to-G base editor
138501SaABE8e-dimerA-to-G base editor
138502SaKKH-ABE8eA-to-G base editor
138503SaKKH-ABE8e-dimerA-to-G base editor
138504LbABE8eA-to-G base editor
138505LbABE8e-dimerA-to-G base editor
138506enAsABE8eA-to-G base editor
138507enAsABE8e-dimerA-to-G base editor
138508LbABE8e(TadA-8e V106W)A-to-G base editor
138509SaABE8e(TadA V106W)A-to-G base editor
138510pBT120a17P1
138511pBT44cP2
138512pBT128cP2
138513pBT138b-pro1P3h
138514pBT138b-proAP3g
138515pBT138b-proBP3f
138516pBT138b-proDP3e
138517pMR-A-3P3a
138518pMR-A-4P3b
138519pMR-A-5P3c
138520pMR-A-6P3d
138521pBT29phage cloning
138522pBT100.164phage cloning
138523pBT114phage cloning

Antibodies from Article