Improved bacterial recombineering by parallelized protein discovery.
Wannier TM, Nyerges A, Kuchwara HM, Czikkely M, Balogh D, Filsinger GT, Borders NC, Gregg CJ, Lajoie MJ, Rios X, Pal C, Church GM
Proc Natl Acad Sci U S A. 2020 Jun 16;117(24):13689-13698. doi: 10.1073/pnas.2001588117. Epub 2020 May 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 128969 | pORTMAGE312B | Broad-host-range KanR plasmid expressing PapRecT and EcMutL_E32K with Toluic acid induction |
| 138474 | pORTMAGE-Ec1 | Enables high-efficiency recombineering in E. coli |
| 138475 | pORTMAGE-Pa1 | Enables high-efficiency recombineering in P. aeruginosa |