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Multiplexed capture of spatial configuration and temporal dynamics of locus-specific 3D chromatin by biotinylated dCas9.

Liu X, Chen Y, Zhang Y, Liu Y, Liu N, Botten GA, Cao H, Orkin SH, Zhang MQ, Xu J
Genome Biol. 2020 Mar 5;21(1):59. doi: 10.1186/s13059-020-01973-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
138417CAPTURE1-1_pLVX-EF1a-BirA-P2A-FB-dCas9-IRES-zsGreen1CAPTURE1.1 vector containing BirA-V5-His, P2A, FB-dCas9, IRES and zsGreen1
138418CAPTURE2_pLVX-EF1a-dCas9-CBio-IRES-zsGreen1CAPTURE2.0 vector containing dCas9-CBio, IRES and zsGreen1
138419CAPTURE2_pLVX-EF1a-NBio-dCas9-IRES-zsGreen1CAPTURE2.0 vector containing NBio-dCas9, IRES and zsGreen1

Antibodies from Article