RNA-Guided Recombinase-Cas9 Fusion Targets Genomic DNA Deletion and Integration.

Standage-Beier K, Brookhouser N, Balachandran P, Zhang Q, Brafman DA, Wang X
CRISPR J. 2019 Aug;2:209-222. doi: 10.1089/crispr.2019.0013. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
138257	pRS424-ysg(G:H)	Expression of GFP-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequence to be used in yeast dual reporter system
138258	pRS424-ysg(NT)	Expression of a non-targeting sgRNA to be used as a control in a yeast dual reporter system
138259	pUC19-hsg(G:H)	Expression of humanized mCherry-targeting sgRNAs to the left (G) and right (H) of iCas9 recognition sequences to be used in Traffic Light reporter system
138260	pUC19-hsg(NT:NT)	Expression of a non-targeting sgRNA to the left and right of iCas9 recognition site to be used as a control in Traffic Light reporter system
138261	pSB1C3-FeGFP	Donor-eGFP vector to be used in plasmid to plasmid integration reporter assay. eGFP is conditionally expressed when integrated at iCas9-sites on acceptor plasmid
138262	pSB1C3-hsg(M)	Subcloning and expression of mCherry-targeting sgRNA M for use in Traffic Light reporter system
138263	pSB1C3-ysg(NT)	Subcloning and expression of non-targeting sgRNA for use in yeast dual reporter system.
138264	pMG	Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.
138265	pUC19-EAMP	mCherry acceptor plasmid with iCas9 target site upstream of mCherry to be used in plasmid to plasmid integration reporter assay.
138266	p415:iCas9	Expression of mTN3-GGS6-dCas9 (iCas9) in yeast.
138267	pEF:iCas9	Expression of mTN3-GGS6-dCas9 (iCas9) in human.