AKAP95 Organizes a Nuclear Microdomain to Control Local cAMP for Regulating Nuclear PKA.
Clister T, Greenwald EC, Baillie GS, Zhang J
Cell Chem Biol. 2019 Jun 20;26(6):885-891.e4. doi: 10.1016/j.chembiol.2019.03.003. Epub 2019 Apr 11.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
138254 | pcDNA3.1(+)-AKAP95-FRB | Full-length AKAP95 fused to FRB (rapamycin-binding domain). Used in conjunction with pcDNA3-FKBP-ICUE3-NLS to monitor cAMP dynamics within AKAP95 nanodomain. |
138255 | pcDNA3-FKBP-ICUE3-NLS | FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain. |