The gammaherpesviral TATA-box-binding protein directly interacts with the CTD of host RNA Pol II to direct late gene transcription.
Castaneda AF, Didychuk AL, Louder RK, McCollum CO, Davis ZH, Nogales E, Glaunsinger BA
PLoS Pathog. 2020 Sep 4;16(9):e1008843. doi: 10.1371/journal.ppat.1008843. eCollection 2020 Sep.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138420 | pcDNA4/TO-ORF24 1-201-3xFLAG | Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells |
| 138421 | pcDNA4/TO-ORF24 1-271-3xFLAG | Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells |
| 138422 | pcDNA4/TO-ORF24 1-133-3xFLAG | Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells |
| 138423 | pcDNA4/TO-ORF24-3xFLAG | Expresses full-length ORF24 with a C-terminal 3xFLAG tag in mammalian cells |
| 138424 | pcDNA4/TO-ORF24 202-752-3xFLAG | Expresses truncated ORF24 with a C-terminal 3xFLAG tag in mammalian cells |
| 138425 | pcDNA4/TO-ORF24 3L_A-3xFLAG | Expresses full-length ORF24 3L_A with a C-terminal 3xFLAG tag in mammalian cells |
| 138426 | pcDNA4/TO-ORF24 L73A-3xFLAG | Expresses full-length ORF24 L73A with a C-terminal 3xFLAG tag in mammalian cells |
| 138427 | pcDNA4/TO-ORF24 L74A-3xFLAG | Expresses full-length ORF24 L74A with a C-terminal 3xFLAG tag in mammalian cells |
| 138428 | pcDNA4/TO-ORF24 L75A-3xFLAG | Expresses full-length ORF24 L75A with a C-terminal 3xFLAG tag in mammalian cells |
| 138429 | pcDNA4/TO-ORF24 L73A_L75A-3xFLAG | Expresses full-length ORF24 L73A/L75A with a C-terminal 3xFLAG tag in mammalian cells |
| 138430 | pcDNA4/TO-ORF24 L74A_L75A-3xFLAG | Expresses full-length ORF24 L74A/L75A with a C-terminal 3xFLAG tag in mammalian cells |
| 138431 | pcDNA4/TO-ORF24 R68A-3xFLAG | Expresses full-length ORF24 R68A with a C-terminal 3xFLAG tag in mammalian cells |
| 138432 | pcDNA4/TO-ORF24 R72A-3xFLAG | Expresses full-length ORF24 R72A with a C-terminal 3xFLAG tag in mammalian cells |
| 138433 | pcDNA4/TO-ORF24 L73A_L74A-3xFLAG | Expresses full-length ORF24 L73A/L74A with a C-terminal 3xFLAG tag in mammalian cells |
| 138434 | pcDNA4/TO-UL87-2xStrep | Expresses full-length UL87 with a C-terminal 2xStrep tag in mammalian cells |
| 138435 | pcDNA4/TO-mu24-2xStrep | Expresses full-length mu24 with a C-terminal 2xStrep tag in mammalian cells |
| 138436 | pcDNA4/TO-BcRF1-2xStrep | Expresses full-length BcRF1 with a C-terminal 2xStrep tag in mammalian cells |
| 138437 | pcDNA4/TO-UL87 3L_A-2xStrep | Expresses full-length UL87 3L_A with a C-terminal 2xStrep tag in mammalian cells |
| 138438 | pcDNA4/TO-mu24 3L_A-2xStrep | Expresses full-length mu24 3L_A with a C-terminal 2xStrep tag in mammalian cells |
| 138439 | pcDNA4/TO-BcRF1 3L_A-2xStrep | Expresses full-length BcRF1 3L_A with a C-terminal 2xStrep tag in mammalian cells |
| 138440 | pcDNA4/TO-ORF24 3L_A-2xStrep | Expresses full-length ORF24 3L_A with a C-terminal 2xStrep tag in mammalian cells |
| 138441 | pcDNA4/TO-ORF24 1-191-2xStrep | Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells |
| 138442 | pcDNA4/TO-ORF24 1-201-2xStrep | Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells |
| 138443 | pcDNA4/TO-ORF24 1-226-2xStrep | Expresses truncated ORF24 with a C-terminal 2xStrep tag in mammalian cells |
| 138444 | pcDNA4/TO-mu24 1-181-2xStrep | Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells |
| 138445 | pcDNA4/TO-mu24 1-191-2xStrep | Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells |
| 138446 | pcDNA4/TO-mu24 1-216-2xStrep | Expresses truncated mu24 with a C-terminal 2xStrep tag in mammalian cells |
| 138447 | pcDNA4/TO-BcRF1 1-168-2xStrep | Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells |
| 138448 | pcDNA4/TO-BcRF1 1-178-2xStrep | Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells |
| 138449 | pcDNA4/TO-BcRF1 1-203-2xStrep | Expresses truncated BcRF1 with a C-terminal 2xStrep tag in mammalian cells |
| 138450 | pcDNA4/TO-UL87 1-238-2xStrep | Expresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells |
| 138451 | pcDNA4/TO-UL87 1-248-2xStrep | Expresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells |
| 138452 | pcDNA4/TO-UL87 1-273-2xStrep | Expresses truncated UL87 with a C-terminal 2xStrep tag in mammalian cells |
| 138453 | pcDNA4/TO-mu24-ORF24-2xStrep | Expresses mu24-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells |
| 138454 | pcDNA4/TO-BcRF1-ORF24-2xStrep | Expresses BcRF1-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells |
| 138455 | pcDNA4/TO-UL87-ORF24-2xStrep | Expresses UL87-ORF24 chimera with a C-terminal 2xStrep tag in mammalian cells |
| 138464 | pGEX4T1-ORF24-NTD | Expresses GST-tagged ORF24-NTD in bacteria |
| 138465 | pMAL-c2X-ORF24-NTD | Expresses MBP-tagged ORF24-NTD in bacteria |
| 138466 | pMAL-c2X-ORF24-NTD-3L_A | Expresses MBP-tagged ORF24-NTD 3L_A in bacteria |
| 138467 | p6H-SUMO3-ORF24-NTD-Strep | Expresses SUMO3-tagged ORF24-NTD-Strep in bacteria |
| 138468 | pGEX4T1-Rpb1-linker | Expresses GST-tagged Rpb1 linker in bacteria |
| 138470 | pQLink-GST-2xCTD | Expresses GST-tagged 2xCTD heptapeptide repeat in bacteria |
| 138471 | pQLink-GST-4xCTD | Expresses GST-tagged 4xCTD heptapeptide repeat in bacteria |
| 138472 | pQLink-GST-10xCTD | Expresses GST-tagged 10xCTD heptapeptide repeat in bacteria |
| 138484 | pGEX4T1-Rpb4/7 | Expresses GST-tagged Rpb4 and His-tagged Rpb7 in bacteria |