An expanded library of orthogonal split inteins enables modular multi-peptide assemblies.
Pinto F, Thornton EL, Wang B
Nat Commun. 2020 Mar 23;11(1):1529. doi: 10.1038/s41467-020-15272-2.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 138167 | pFP.R265 | Expresses the split mCherry reporter interrupted by the SspDnaB M86 artificially mini-intein (in cis) |
| 138175 | pFP.R400 | Expresses the split mCherry reporter interrupted by the chimeric Npu/SspDnaE intein (in cis) |
| 138179 | pFP.R267 | Expresses the split mCherry reporter interrupted by the gp41-1 intein (in cis) |
| 138180 | pFP.R268 | Expresses the split mCherry reporter interrupted by the gp41-8 intein (in cis) |
| 138181 | pFP.R269 | Expresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis) |
| 138219 | pFP.R270 | Expresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis) |
| 138220 | pFP.R271 | Expresses the split mCherry reporter interrupted by the SspDnaX artificially mini-intein (in cis) |
| 138221 | pFP.R272 | Expresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis) |
| 138222 | pFP.R401 | Expresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis) |
| 138223 | pFP.R274 | Expresses the split mCherry reporter interrupted by the TvoVMA intein (in cis) |
| 138224 | pFP.R275 | Expresses the split mCherry reporter interrupted by the PhoRadA intein (in cis) |
| 138225 | pFP.R355 | Expresses the split mCherry reporter interrupted by the Cro-RIR1 artificially mini-intein (in cis) |
| 138226 | pFP.R356 | Expresses the split mCherry reporter interrupted by the Cro-RPB2 intein (in cis) |
| 138227 | pFP.R357 | Expresses the split mCherry reporter interrupted by the Cro-Top2 artificially mini-intein (in cis) |
| 138228 | pFP.R358 | Expresses the split mCherry reporter interrupted by the CIV-RIR1 artificially mini-intein (in cis) |
| 138229 | pFP.R359 | Expresses the split mCherry reporter interrupted by the CP-Thy1 artificially mini-intein (in cis) |
| 138230 | pFP.R360 | Expresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis) |
| 138231 | pFP.R361 | Expresses the split mCherry reporter interrupted by the BsuP-RIR1 artificially mini-intein (in cis) |
| 138232 | pFP.R362 | Expresses the split mCherry reporter interrupted by the CbP-RNR artificially mini-intein (in cis) |
| 138233 | pFP.R363 | Expresses the split mCherry reporter interrupted by the Ckl-Ter artificially mini-intein (in cis) |
| 138234 | pFP.R364 | Expresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis) |
| 138235 | pFP.R365 | Expresses the split mCherry reporter interrupted by the EP-Pri artificially mini-intein (in cis) |
| 138236 | pFP.R366 | Expresses the split mCherry reporter interrupted by the LLP-Pol intein (in cis) |
| 138237 | pFP.R367 | Expresses the split mCherry reporter interrupted by the LP-Hel intein (in cis) |
| 138238 | pFP.R368 | Expresses the split mCherry reporter interrupted by the MP-B-DnaB artificially mini-intein (in cis) |
| 138239 | pFP.R369 | Expresses the split mCherry reporter interrupted by the MP-K-gp53 intein (in cis) |
| 138240 | pFP.R370 | Expresses the split mCherry reporter interrupted by the MP-C-gp206 artificially mini-intein (in cis) |
| 138241 | pFP.R371 | Expresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis) |
| 138242 | pFP.R372 | Expresses the split mCherry reporter interrupted by the PP-Phi artificially mini-intein (in cis) |
| 138243 | pFP.R373 | Expresses the split mCherry reporter interrupted by the SaP-dpol intein (in cis) |
| 138244 | pFP.R374 | Expresses the split mCherry reporter interrupted by the SaP-Hel artificially mini-intein (in cis) |
| 138245 | pFP.R375 | Expresses the split mCherry reporter interrupted by the NrdA-2 intein (in cis) |
| 138246 | pFP.R376 | Expresses the split mCherry reporter interrupted by the Pfu-RIR1-1 artificially mini-intein (in cis) |
| 138247 | pFP.R377 | Expresses the split mCherry reporter interrupted by the Mja-KlbA intein (in cis) |
| 138248 | pFP.E222 | Three input / three output logic circuit plasmid - Expresses different intein split ECFs from different inducible promoters |
| 138249 | pFP.E227 | Reporter plasmid - Expresses three fluorescent reporters from three different ECF promoters |