Practical method for super-resolution imaging of primary cilia and centrioles by expansion microscopy using an amplibody for fluorescence signal amplification.
Katoh Y, Chiba S, Nakayama K
Mol Biol Cell. 2020 Jul 29:mbcE20040250. doi: 10.1091/mbc.E20-04-0250.
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Plasmids from Article
ID | Plasmid | Purpose |
---|---|---|
138128 | pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody | Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells. |
138129 | pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobody | Expresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells. |
138130 | pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobody | Expresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells. |
138131 | pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobody | Expresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells. |
138132 | pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody | Expresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells. |
138133 | pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobody | Expresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells. |