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Practical method for super-resolution imaging of primary cilia and centrioles by expansion microscopy using an amplibody for fluorescence signal amplification.

Katoh Y, Chiba S, Nakayama K
Mol Biol Cell. 2020 Jul 29:mbcE20040250. doi: 10.1091/mbc.E20-04-0250. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
138128pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobodyExpresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.
138129pGEX6P1-mClover3-anti-rabbit-IgG(Fc)-nanobodyExpresses GST-tagged mClover3-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.
138130pGEX6P1-mCherry-anti-mouse-IgG(kappa)-nanobodyExpresses GST-tagged mCherry-anti-mouse-IgG(kappa)-nanobody in E. coli cells.
138131pGEX6P1-mCherry-anti-rabbit-IgG(Fc)-nanobodyExpresses GST-tagged mCherry-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.
138132pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobodyExpresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.
138133pGEX6P1-EGFP-anti-rabbit-IgG(Fc)-nanobodyExpresses GST-tagged EGFP-anti-rabbit-IgG(Fc)-nanobody in E. coli cells.

Antibodies from Article