Pronuclear Microinjection during S-Phase Increases the Efficiency of CRISPR-Cas9-Assisted Knockin of Large DNA Donors in Mouse Zygotes.
Abe T, Inoue KI, Furuta Y, Kiyonari H
Cell Rep. 2020 May 19;31(7):107653. doi: 10.1016/j.celrep.2020.107653.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 137925 | R26-H2B-EGFP HR donor vector | R0SA26 knock-in vector with homologous recombination-based method |
| 137926 | R26-EGFP MMEJ donor vector | R0SA26 knock-in vector with microhomology-mediated end-joining(MMEJ)-based method |
| 137927 | R26-EGFP HMEJ donor vector | ROSA26 knock-in vector with homology-mediated end joining(HMEJ)-based method |
| 137928 | R26-H2B-mCherry HR donor vector | R0SA26 knock-in vector with homologous recombination-based method |