Computation-guided optimization of split protein systems.
Dolberg TB, Meger AT, Boucher JD, Corcoran WK, Schauer EE, Prybutok AN, Raman S, Leonard JN
Nat Chem Biol. 2021 May;17(5):531-539. doi: 10.1038/s41589-020-00729-8. Epub 2021 Feb 1.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 137826 | FKBP-CD28TMD-NTEVp(WT) | MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV protease |
| 137827 | FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA | MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor |
| 137828 | FKBP-CD28TMD-NTEVp(H75S) | MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV protease |
| 137829 | FKBP-CD28TMD-NTEVp(H75E) | MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease |
| 137830 | FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA | MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor |
| 137831 | FKBP-NTEVp(WT) | Cytosolic split-TEV protease chain with FKBP Rapamycin sensing domain, N-terminal WT TEV protease |
| 137832 | FRB-CTEVp(WT) | Cytosolic split-TEV protease chain with FRB Rapamycin sensing domain, C-terminal WT TEV protease |
| 137836 | 3NES-CS(G)-tTA-CS(G)-3NES | Soluble tTA transcription factor flanked by N and C terminal TEV protease G cleavage sequences and 3 nuclear export sequences |