Improved LbCas12a variants with altered PAM specificities further broaden the genome targeting range of Cas12a nucleases.

Toth E, Varga E, Kulcsar PI, Kocsis-Jutka V, Krausz SL, Nyeste A, Welker Z, Huszar K, Ligeti Z, Talas A, Welker E
Nucleic Acids Res. 2020 Feb 28. pii: 5763098. doi: 10.1093/nar/gkaa110. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
137714	pTE3646	Expresses LbCas12a RVRR variant in mammalian cells.
137715	pVE13300	Expresses impLbCas12a in mammalian cells.
137716	pTE3987	Expresses dLbCas12a-RVRR-BE in mammalian cells.
137717	pTE2690	Expresses dimpLbCas12a-SunTag in mammalian cells.