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iFLinkC: an iterative functional linker cloning strategy for the combinatorial assembly and recombination of linker peptides with functional domains.

Grawe A, Ranglack J, Weyrich A, Stein V
Nucleic Acids Res. 2020 Jan 11. pii: 5700546. doi: 10.1093/nar/gkz1210. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
137090pL2-GiFLinkC Linker cloning
137091pL2-G2iFLinkC Linker cloning
137092pL2-G3iFLinkC Linker cloning
137093pL2-GPGiFLinkC Linker cloning
137094pL2-GPPPGiFLinkC Linker cloning
137095pL2-GGSGiFLinkC Linker cloning
137096pL2-GSPAGiFLinkC Linker cloning
137097pL2-(GGS)2GiFLinkC Linker cloning
137098pL2-(GGS)3GiFLinkC Linker cloning
137099pL2-(GGS)4GSGiFLinkC Linker cloning
137100pL2-G(P)7GiFLinkC Linker cloning
137101pL2-G(TP)4TGiFLinkC Linker cloning
137102pL2-GGASPAGGiFLinkC Linker cloning
137103pL2-GGASPAAPAPAGiFLinkC Linker cloning
137104pL2-GGA(EAAAK)2AGGiFLinkC Linker cloning
137105pL2-G(P)10GiFLinkC Linker cloning
137106pL2- GL(GGGS)3ETGiFLinkC Linker cloning
137107pFD-FRBiFLinkC Linker cloning
137108pFD-FKBP12iFLinkC Linker cloning
137109pFD-NIa-Protease-TVMViFLinkC Linker cloning
137110pFD-MBP-CS(TEV)-StrepTag-II-AI(TVMV)iFLinkC Linker cloning
137111pFD-AI(TVMV)iFLinkC Linker cloning
137112pFD-FN3iFLinkC Linker cloning
137113pFD-ePDZ-B1iFLinkC Linker cloning
137114pFLinkC-XEiFLinkC Linker cloning
137115pFLinkC-AI(GPG)-FKBP12(TP11)-FRB(GPG)-TVMVProtease Switch Clone ID: 1-A9
137116pFLinkC-AI(GPG)-FKBP12(EAAAK16)-FRB(PAS5)-TVMVProtease Switch Clone ID: 1-C9
137117pFLinkC-AI(G3)-PDZ(GPG-G)FN3(GGS4)-TVMVProtease Switch Clone ID: 2-A5
137118pFLinkC-AI(GPG)-FKBP12(P9)-FRB(GPG)-TVMVProtease Switch Clone ID: 2-B3

Antibodies from Article