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An Engineered Cas-Transposon System for Programmable and Site-Directed DNA Transpositions.

Chen SP, Wang HH
CRISPR J. 2019 Dec;2(6):376-394. doi: 10.1089/crispr.2019.0030. Epub 2019 Nov 19. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
137079pET-Himar-dCas9Himar-dCas9 gene with C-terminal 6x-His tag for protein purification
137080pHdCas9Expression of Himar-dCas9 in bacteria
137081pHimar6bacterial Himar1 mini-transposon donor plasmid
138414pET-Himar-dCas9-mammalianProtein purification of Himar-dCas9 with 3x-FLAG, SV40 NLS, and 6x-His tag
138415pHdCas9-mammalianExpression of Himar-dCas9 in mammalian cells

Antibodies from Article