Genetic screens in isogenic mammalian cell lines without single cell cloning.
DeWeirdt PC, Sangree AK, Hanna RE, Sanson KR, Hegde M, Strand C, Persky NS, Doench JG
Nat Commun. 2020 Feb 6;11(1):752. doi: 10.1038/s41467-020-14620-6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 133456 | pXPR_213 | H1 promoter expresses customizable Saur-guide; EF1a promoter expresses SpyoCas9 and 2A site provides blasticidin resistance |
| 133457 | pXPR_212 | U6 promoter expresses customizable Spyo-guide; EFS promoter expresses SaurCas9 and 2A site provides puromycin resistance |
| 133458 | pRDA_186 | U6 promoter expresses customizable Spyo-guide; PGK promoter expresses blasticidin resistance and 2A site provides EGFP |
| 133459 | pRDA_118 | U6 promoter expresses customizable Spyo-guide; EF1a promoter provides puromycin resistance. This vector is a derivative of the lentiGuide vector, with minor modifications to the tracrR |
| 133460 | pRDA_103 | H1 promoter with two Tet operator (TetO) sites expresses customizable Spyo-guide; short EF1a promoter (EFS) expresses SaurCas9, 2A provides TetR, and 2A provides blasticidin resistance. |
| 133461 | pXPR_124 | EF1a promoter expresses SpyoCas9 and P2A provides EGFP |
| 136986 | Human sgRNA library Gattinara in pRDA_118 backbone | Expresses sgRNAs targeting human genes |
| 136987 | Mouse sgRNA library Gouda in pRDA_118 backbone | Expresses sgRNAs targeting mouse genes |