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Deconstructing Cell-Free Extract Preparation for in Vitro Activation of Transcriptional Genetic Circuitry.

Silverman AD, Kelley-Loughnane N, Lucks JB, Jewett MC
ACS Synth Biol. 2019 Feb 15;8(2):403-414. doi: 10.1021/acssynbio.8b00430. Epub 2019 Jan 29. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
136942pJBL7010Constitutive sfGFP expression (high), bacterial promoter
136943pJBL7004Malachite green aptamer, T7 promoter
136944pJBL7005Malachite green aptamer with stability hairpin, T7 promoter
136945pJBL7006Malachite green aptamer, bacterial promoter
136946pJBL7007Malachite green aptamer with stability hairpin, bacterial promoter

Antibodies from Article