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GO: a functional reporter system to identify and enrich base editing activity.

Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE
Nucleic Acids Res. 2020 Feb 29. pii: 5766653. doi: 10.1093/nar/gkaa124. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
136890pRRL-GFPGO-PGK-NeoLentivirus for base editing activatable GFP expression in mammalian cells
136891pRRL-GFPGO2(NGG)-PGK-NeoLentivirus for base editing activatable GFP expression in mammalian cells
136892pRRL-GFPGO2(NGT)-PGK-NeoLentivirus for base editing activatable GFP expression in mammalian cells
136893pRRL-GFPGO2(NGC)-PGK-NeoLentivirus for base editing activatable GFP expression in mammalian cells
136895pRRL-mU6-GFPGO-IRES-mScarletILentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO2.
136896pRRL-mU6-GFPGO2-IRES-mScarletILentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.
136897pRRL-mU6-GFPGO-IRES-mScarletI-PGK-NeoLentivirus for base editing activatable GFP expression in mammalian cells. All-in-one vector with sgGO.
136898pRRL-GFPAdGO-PGK-NeoLentivirus for base editing activatable GFP expression in mammalian cells
136899pRRL-GFPAdGO2-PGK-NeoLentivirus for base editing activatable GFP expression in mammalian cells
136900pLenti-FNLSNG-P2A-PuroLentivirus for constitutive expression of FNLSNG in mammalian cells (codon optimized)
136901pLenti-FNLSHF1-P2A-PuroLentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)
136902pLenti-FNLSHiFi-P2A-PuroLentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)
136903pLenti-FNLSHiFiNG-P2A-PuroLentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)
136904pRRL-mU6-mScarletGO-P2A-Blas-PGK-NeoLentivirus for base editing activatable mScarletI expression in mammalian cells. All-in-one vector with sgmScarletGO.
136905pLenti-mU6-Luc2GO-PGK-NeoLentivirus for base editing activatable Luciferase2 expression in mammalian cells. All in one vector with sgLuc2GO.
136906pLenti-hU6-mU6-BlasGOLentivirus for base editing activatable Blasticidin resistance gene expression in mammalian cells. All in one vector with sgBlasGO.
136907pLenti-hU6-mU6-Cre2GOLentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.
136908LRT2B-GOLentivirus expressing GO sgRNA
136909LRT2B-GO2Lentivirus expressing GO2 sgRNA
136910LRT2B-AdGOLentivirus expressing AdGO sgRNA
136911LRT2B-AdGO2Lentivirus expressing AdGO2 sgRNA

Antibodies from Article