Combining Cell Fate Reprogramming and Protein Engineering to Study Transcription Factor Functions.
Adrian-Segarra JM, Weigel B, Mall M
Methods Mol Biol. 2021;2352:227-236. doi: 10.1007/978-1-0716-1601-7_15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136884 | TetO-FUW-FLAG-NLS-EnR-MCS_N-term | Doxycycline-inducible vector backbone containing the engrailed (EnR) repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS) |
| 136885 | TetO-FUW-MCS-EnR_C-term | Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the engrailed (EnR) repressor domain and a STOP codon |
| 136886 | TetO-FUW-FLAG-NLS-VP64-MCS_N-term | Doxycycline-inducible vector backbone containing the VP64 activator domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS) |
| 136887 | TetO-FUW-MCS-VP64_C-term | Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the VP64 activator domain and a STOP codon |
| 136888 | TetO-FUW-FLAG-NLS-KRAB-MCS_N-term | Doxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS) |
| 136889 | TetO-FUW-MCS-KRAB_C-term | Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the KRAB repressor domain and a STOP codon |