The Autophagy-Related Beclin-1 Protein Requires the Coiled-Coil and BARA Domains To Form a Homodimer with Submicromolar Affinity.
Ranaghan MJ, Durney MA, Mesleh MF, McCarren PR, Garvie CW, Daniels DS, Carey KL, Skepner AP, Levine B, Perez JR
Biochemistry. 2017 Dec 26;56(51):6639-6651. doi: 10.1021/acs.biochem.7b00936. Epub 2017 Dec 14.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136684 | BPV00500 pET21GG2 His(6)-TF-BECN(1-450) | Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor) |
| 136685 | BPV00554 pET21GG2-His(6)-BECN(248-450) | Expresses the Beclin-1 ECD/BARA domain (a.a. 248-450) with a N-terminal hexa-Histidine tag |
| 136686 | BPV00605 pET21GG2-His(6)-BECN(1-265) | Expresses the Beclin-1 IDR+CC domains (a.a. 1-265) with a N-terminal hexa-Histidine tag |
| 136688 | BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA] | Expresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface. |