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A set of monomeric near-infrared fluorescent proteins for multicolor imaging across scales.

Matlashov ME, Shcherbakova DM, Alvelid J, Baloban M, Pennacchietti F, Shemetov AA, Testa I, Verkhusha VV
Nat Commun. 2020 Jan 13;11(1):239. doi: 10.1038/s41467-019-13897-6. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
136556pemiRFP670-N1A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)
136557pmiRFP680-N1A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP680 (another name miRFP713V254C)
136558pemiRFP703-N1A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)
136559pmiRFP713-N1A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP713
136560pmiRFP720-N1A cytoplasmic expression of a monomeric phytochrome-based near-infrared fluorescent protein miRFP720
136561pmiRFP713-tubulinHuman alpha-tubulin labeling with a near-infrared fluorescent protein miRFP713
136562pLifeAct-miRFP713F-actin labeling with a near-infrared fluorescent protein miRFP713
136563pemiRFP703-clathrinClathrin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)
136564pemiRFP703-tubulinHuman alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)
136565pemiRFP703-myosinLC-Myosin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)
136566pvimentin-emiRFP703Vimentin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)
136567pH2B-emiRFP703Histone 2B labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)
136568pzyxin-emiRFP703Zyxin labeling with near-infrared fluorescent protein emiRFP703 (enhanced miRFP703)
136569pvimentin-miRFP713Vimentin labeling with near-infrared fluorescent protein miRFP713
136570pLAMP1-emiRFP670LAMP1 (Lysosome Membrane) labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)
136571pH2B-emiRFP670Histone 2B labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)
136572pvimentin-emiRFP670Vimentin labeling with near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)
136573pemiRFP670-tubulinHuman alpha-tubulin labeling with a near-infrared fluorescent protein emiRFP670 (enhanced miRFP670)
136574pmiRFP720-tubulinHuman alpha-tubulin labeling with a near-infrared fluorescent protein miRFP720
136575pmiRFP680-tubulinHuman alpha-tubulin labeling with a near-infrared fluorescent protein miRFP680

Antibodies from Article