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Sporadic activation of an oxidative stress-dependent NRF2-p53 signaling network in breast epithelial spheroids and premalignancies.

Pereira EJ, Burns JS, Lee CY, Marohl T, Calderon D, Wang L, Atkins KA, Wang CC, Janes KA
Sci Signal. 2020 Apr 14;13(627). pii: 13/627/eaba4200. doi: 10.1126/scisignal.aba4200. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
136519pEN_TTmiRC2_3xflag_LuciferaseUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK
136520pEN_TTmiRC2_p53DDUsed as a donor vector containing N-term 3xFLAG to clone into pSLIK
136521pEN_TTmiRc2_BirAUsed as a donor vector to clone into pSLIK
136522pEN_TTmiRC2_3xflag_NRF2(RR)Used as a donor vector containing N-term 3xFLAG to clone into pSLIK
136523pEN_TTmiRc2_BirA_CDKN1AUsed as a donor vector to clone into pSLIK
136524pEN_TTmiRc2_BirA_NRF2Used as a donor vector to clone into pSLIK
136525pEN_TTmiRc2_p53(R280K)_V5Used as a donor vector to clone into pSLIK
136526pEN_TTmiRc2_3xflag_CHEK2Used as a donor vector containing N-term 3xFLAG to clone into pSLIK
136527pEN_TTmiRc2_3xflag_NRF2Used as a donor vector containing N-term 3xFLAG to clone into pSLIK
136528pSLIK_hygro_3xflag_LuciferaseLentiviral expression vector for an inducible 3xflag-tagged Luciferase
136533pSLIK_Zeo_3xflag_LuciferaseLentiviral expression vector for an inducible 3xflag-tagged Luciferase
136534pSLIK Zeo p53DDLentiviral expression vector for an inducible p53DD
136535pSLIK_hygro_3xflag_NRF2(RR)Lentiviral expression vector for an inducible 3xflag-tagged NRF2(a1584c_a1586t_a1589g)
136536pSLIK_neo_3xflag_CHEK2Lentiviral expression vector for an inducible 3xflag-tagged CHEK2
136537pSLIK_hygro_BirALentiviral expression vector for an inducible BirA
136538pSLIK_hygro_BirA_CDKN1ALentiviral expression vector for an inducible BirA-CDKN1A
136539pSLIK_hygro_BirA_NRF2Lentiviral expression vector for an inducible BirA-NRF2
136540pSLIK_hygro_p53(R280K)_V5Lentiviral expression vector for an inducible p53(R280K)-V5
136579pBABE_hygro_mRFP1_NRF2mammalian expression of mRFP1-tagged human NRF2
136580pBABE_hygro_mRFP1_NRF2(C506S, L4A)mammalian expression of mRFP1-tagged human NRF2(C506S, Leuzip mut)
136581tet-pLKO.1_puro_shJUND #1Expresses an inducible short hairpin targeting human JUND sequence
136583tet-pLKO.1_puro_shJUND #2Expresses an inducible short hairpin targeting human JUND sequence
136584tet_pLKO.1_puro_shNRF2 #1Expresses an inducible short hairpin targeting human NRF2 sequence
136585tet_pLKO.1_puro_shNRF2 #2Expresses an inducible short hairpin targeting human NRF2 sequence
136587tet_pLKO.1_puro_shLucExpresses an inducible short hairpin targeting firefly luciferase sequence
136588pLXSN_HPV16E7∆DLYCRetroviral vector for expression of RB-binding mutant of HPVE7

Antibodies from Article