Anti-CRISPR AcrIIA5 Potently Inhibits All Cas9 Homologs Used for Genome Editing.
Garcia B, Lee J, Edraki A, Hidalgo-Reyes Y, Erwood S, Mir A, Trost CN, Seroussi U, Stanley SY, Cohn RD, Claycomb JM, Sontheimer EJ, Maxwell KL, Davidson AR
Cell Rep. 2019 Nov 12;29(7):1739-1746.e5. doi: 10.1016/j.celrep.2019.10.017.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136513 | pEJS1004-pCSDest2-AcrIIA5-NLS-FLAG | Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells |
| 136514 | pEJS1005-Lenti-mammalian c.o.AcrIIA5-FLAG-NLS-HygR | Expresses type II-A anti-CRISPR protein AcrIIA5 in mammalian cells in a lentiviral vector |