Ultrasensitive Genetically Encoded Indicator for Hydrogen Peroxide Identifies Roles for the Oxidant in Cell Migration and Mitochondrial Function.
Pak VV, Ezerina D, Lyublinskaya OG, Pedre B, Tyurin-Kuzmin PA, Mishina NM, Thauvin M, Young D, Wahni K, Martinez Gache SA, Demidovich AD, Ermakova YG, Maslova YD, Shokhina AG, Eroglu E, Bilan DS, Bogeski I, Michel T, Vriz S, Messens J, Belousov VV
Cell Metab. 2020 Mar 3;31(3):642-653.e6. doi: 10.1016/j.cmet.2020.02.003.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136464 | pLifeAct-HyPer7 | Mammalian expression of F-actin targeted ultrasensitive hydrogen peroxide indicator HyPer7 fused with LifeAct peptide for optical imaging |
| 136465 | pCMV-HyPer7-MEM | Mammalian expression of cytosolic side of plasma membrane targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging |
| 136466 | pCS2+HyPer7 | Mammalian expression of non-targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging |
| 136467 | pCS2+HyPer7-NES | Mammalian expression of cytoplasm targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging |
| 136468 | pCS2+HyPer7-NLS | Mammalian expression of nucleus targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging |
| 136469 | pCS2+IMS-HyPer7 | Mammalian expression of mitochondrial intermembrane space targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging |
| 136470 | pCS2+MLS-HyPer7 | Mammalian expression of mitochondrial matrix targeted ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging |
| 141071 | pQE30-HyPer7 | Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging |