A Versatile Protocol to Generate Translocations in Yeast Genomes Using CRISPR/Cas9.

Agier N, Fleiss A, Delmas S, Fischer G
Methods Mol Biol. 2021;2196:181-198. doi: 10.1007/978-1-0716-0868-5_14. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
136303	pAEF6	pAEF5 plasmid with two gRNA inserted
136305	pAEF5	Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss