siRNA carrying an (E)-vinylphosphonate moiety at the 5 end of the guide strand augments gene silencing by enhanced binding to human Argonaute-2.
Elkayam E, Parmar R, Brown CR, Willoughby JL, Theile CS, Manoharan M, Joshua-Tor L
Nucleic Acids Res. 2017 Apr 7;45(6):3528-3536. doi: 10.1093/nar/gkw1171.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 136236 | pFl Strep-sumo-TEV-hAgo2 D597N | Expressing catalytically inactive human Argonaute-2 in insect cells |