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Ragulator and SLC38A9 activate the Rag GTPases through noncanonical GEF mechanisms.

Shen K, Sabatini DM
Proc Natl Acad Sci U S A. 2018 Sep 18;115(38):9545-9550. doi: 10.1073/pnas.1811727115. Epub 2018 Sep 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
136141[pSK581] pRK5 HA-SLC38A9Nmammalian expression of SLC38A9 N term fragment (1-119)
136142[pSK104] pET28 SLC38A9N-Hisbacterial expression of SLC38A9 N term fragment (1-119)
136143[pSK24] pACYCDuet His-c7orf59|HBXIPbacterial expression of c7orf59 and HBXIP
136144[pSK122] pETDuet GST-p18(delta5)bacterial expression of GST-p18
136145[pSK131] pCOLADuet p14|MP1bacterial expression of p14 and MP1

Antibodies from Article