A tunable orthogonal coiled-coil interaction toolbox for engineering mammalian cells.
Lebar T, Lainscek D, Merljak E, Aupic J, Jerala R
Nat Chem Biol. 2020 Jan 6. pii: 10.1038/s41589-019-0443-y. doi: 10.1038/s41589-019-0443-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135982 | pcDNA3-His:dCas9:nls:10P3 | Expresses dCas9 fused to 10 copies of the P3 coiled coil in mammalian cells |
| 135983 | pcDNA3-P4:gs:nls:VPR | Expresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cells |
| 135984 | pcDNA3-P4:gs:nls:FKBP | Expresses the P4 coiled coil fused to the FKBP rapamycin-inducible domain in mammalian cells |
| 135985 | pcDNA3-Myc:P4:gs:nls:ABI | Expresses the P4 coiled coil fused to the ABI abscisic acid-inducible domain in mammalian cells |
| 135986 | pcDNA3-P4:gs:nls:CIBN | Expresses the P4 coiled coil fused to the CIBN blue light-inducible domain in mammalian cells |
| 135987 | pcDNA3-His:FRB:nls:VPR | Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells |
| 135988 | pcDNA3-His:PYL1:nls:VPR | Expresses the PYL1 abscisic acid-inducible domain fused to the VPR transcriptional activation domain in mammalian cells |
| 135989 | pcDNA3-His:CRY2:nls:VPR | Expresses the CRY2 blue light-inducible domain fused to the VPR transcriptional activation domain in mammalian cells |