krCRISPR: an easy and efficient strategy for generating conditional knockout of essential genes in cells.
Wang B, Wang Z, Wang D, Zhang B, Ong SG, Li M, Yu W, Wang Y
J Biol Eng. 2019 Apr 24;13:35. doi: 10.1186/s13036-019-0150-y. eCollection 2019.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135970 | KO plasmid | Expresses SpCas9, and cloning backbone for sgRNA |
| 135971 | rescue plasmid | expresses copGFP and puromycin resistance genes |