An episomal vector-based CRISPR/Cas9 system for highly efficient gene knockout in human pluripotent stem cells.
Xie Y, Wang D, Lan F, Wei G, Ni T, Chai R, Liu D, Hu S, Li M, Li D, Wang H, Wang Y
Sci Rep. 2017 May 24;7(1):2320. doi: 10.1038/s41598-017-02456-y.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135960 | epiCRISPR | Expresses SpCas9, and cloning backbone for sgRNA |
| 135961 | epiCRISPRn | Expresses SpCas9, and cloning backbone for sgRNA |
| 135962 | epiCRISPRe | Expresses SpCas9, and cloning backbone for sgRNA |
| 135963 | gRNAU6 | Contains tracrRNA-hU6 sequence for double sgRNA cloning |