Engineered BRET-Based Biologic Light Sources Enable Spatio-Temporal Control Over Diverse Optogenetic Systems.
Parag-Sharma K, O'Banion CP, Henry EC, Musicant AM, Cleveland JL, Lawrence DS, Amelio AL
ACS Synth Biol. 2019 Dec 13. doi: 10.1021/acssynbio.9b00277.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135931 | pET20b-NLuc-MBP-6xHis | Expresses NanoLuciferase in bacterial cells. |
| 135932 | pET20b-GpNLuc-6xHis | Expresses the GpNLuc LumiFluor in bacterial cells. |
| 135933 | pET20b-CeNLuc-6xHis | Expresses the CeNLuc LumiFluor in bacterial cells. |
| 135934 | pWXLd-RIEP-CeNLuc | Expresses the CeNLuc LumiFluor in mammalian cells. |
| 135935 | pWXLd-RIEP-GpNLuc | Expresses the GpNLuc LumiFluor in mammalian cells. |
| 135936 | pWXLd-RIEP-OgNLuc | Expresses the OgNLuc LumiFluor in mammalian cells. |
| 135937 | pRP_8xgRNA-FLuc | Firefly luciferase reporter containing 8 copies of a gRNA binding site for light-inducible dCas9 activation. |
| 135938 | pEF1a-T2-NLuc | Expresses NanoLuciferase targeted to the mitochondira in mammalian cells. |
| 135948 | pEF1a-T2-CeNLuc | Expresses the CeNLuc LumiFluor targeted to the mitochondira in mammalian cells. |
| 135949 | pEF1a-T2-GpNLuc | Expresses the GpNLuc LumiFluor targeted to the mitochondira in mammalian cells. |
| 135951 | pEF1a-T2-OgNLuc | Expresses the OgNLuc LumiFluor targeted to the mitochondira in mammalian cells. |
| 135952 | pEF1a-2xSV40_NLS-CeNLuc | Expresses the CeNLuc LumiFluor targeted to the nucleus in mammalian cells. |
| 135953 | pEF1a-2xSV40_NLS-NLuc | Expresses the NanoLuciferase targeted to the nucleus in mammalian cells. |
| 135954 | pLL7.0-iRFP670-micro | Expresses an iLID micro (SspB R73Q)-iRFP670 fusion in mammalian cells. |