A novel system to map protein interactions reveals evolutionarily conserved immune evasion pathways on transmissible cancers.

Flies AS, Darby JM, Lennard PR, Murphy PR, Ong CEB, Pinfold TL, De Luca A, Lyons AB, Woods GM, Patchett AL
Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eaba5031. doi: 10.1126/sciadv.aba5031. Print 2020 Jul. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
135920	pAF112	All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with luciferse and hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
135921	pAF123	All-in-one Sleeping Beauty vector for stable transfection of eukaryotic cells with hygromycin restistance. CMV protomor drives SB100X transposase for transposon integration.
135923	pAF138	All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCitrine fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
135924	pAF139	All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mTagBFP fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
135925	pAF160	All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mAzurite fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
135926	pAF161	All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mCerulean3 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
135927	pAF163	All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mNeptune2 fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
135928	pAF164	All-in-one Sleeping Beauty vector for Expression for Tasmanian devil TNFRSF9-mOrange fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFRSF9).
135929	pAF92	All-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).
135930	pAF197	All-in-one Sleeping Beauty vector for Expression for Tasmanian devil CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. CTLA4).