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Florian Karreth Lab: Karreth lab plasmids

Unpublished

Plasmids from Article

ID Plasmid Purpose
135663cTRE-PtenWTIntroduce Dox-inducible (TRE promoter) wildtype Pten cDNA into (ES) cells by recombination-mediated cassette exchange
135664cTRE-PtenC124SIntroduce Dox-inducible (TREa promoter) C124S-mutant Pten cDNA into (ES) cells by recombination-mediated cassette exchange
135665cTRE-GFPIntroduce Dox-inducible (TRE promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
135666cTRE-GFP-miR30_shRenIntroduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shRenilla into (ES) cells by recombination-mediated cassette exchange
135667cTRE-GFP-miR30_shPten.1523Introduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shPten into (ES) cells by recombination-mediated cassette exchange
135668cTRE-LSL-GFP-IRES-Cas9-sgCR8Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus control-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
135669cTRE-LSL-GFP-IRES-Cas9-sgPtenX1.1Introduce Dox/Cre-inducible (TRE promoter followed by lox-stop-lox) Cas9 cDNA plus Pten-targeting sgRNA into (ES) cells by recombination-mediated cassette exchange
135670cSFFV-GFPIntroduce constitutive (SFFV promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
135671cEF1a-GFPIntroduce constitutive (EF1a promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
135672cEF1a-LSL-GFPIntroduce Cre-inducible (EF1a promoter followed by lox-stop-lox) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
135673pLenti-TRE-GFP-BlastDox-inducible expression (TRE promoter) of GFP cDNA (control for other cDNAs), Blasticidin selection
135674pLenti-TRE-PtenWT-BlastDox-inducible expression (TRE promoter) of wildtype Pten cDNA, Blasticidin selection
135675pLenti-TRE-PtenC124S-BlastDox-inducible expression (TRE promoter) of C124S mutant Pten cDNA, Blasticidin selection
135676pLenti-CMV-PtenWT-PuroConstitutive expression (cMV promoter) of wildtype Pten cDNA, Puromycin selection
135677pLenti-CMV-PtenC124S-PuroConstitutive expression (cMV promoter) of C124S mutant Pten cDNA, Puromycin selection
135678pLenti-CMV-PtenG129E-PuroConstitutive expression (cMV promoter) of G129E mutant Pten cDNA, Puromycin selection
135679pLenti-CMV-PtenY138L-PuroConstitutive expression (cMV promoter) of Y138L mutant Pten cDNA, Puromycin selection
135680pLenti-CMV-PtenK13E-PuroConstitutive expression (cMV promoter) of K13E mutant Pten cDNA, Puromycin selection
135681pLenti-CMV-PtenK13E/K289E-PuroConstitutive expression (cMV promoter) of K13E/K289E mutant Pten cDNA, Puromycin selection
135682pLenti-EF1a-GFP-miRE_shRen-BlastConstitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
135683pLenti-EF1a-GFP-miRE_shRen-PuroConstitutive expression (EF1a promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
135684pLenti-CMV-GFP-miRE_shRen-BlastConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
135685pLenti-CMV-GFP-miRE_shRen-PuroConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
135686pLenti-CMV-tdTomato-miRE_shRen-BlastConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Blasticidin selection
135687pLenti-CMV-tdTomato-miRE_shRen-PuroConstitutive expression (CMV promoter) of tdTomato cDNA plus miRE-embedded shRenilla (control shRNA), Puromycin selection
135688pLenti-CMV-GFP-miRE_shSCR-BlastConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Blasticidin selection
135689pLenti-CMV-GFP-miRE_shSCR-PuroConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded scrambled control shRNA, Puromycin selection
135690pLenti-CMV-GFP-miRE_shTHUMPD3-BlastConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Blasticidin selection
135691pLenti-CMV-GFP-miRE_shTHUMPD3-PuroConstitutive expression (CMV promoter) of GFP cDNA plus miRE-embedded shROSA26 (control shRNA), Puromycin selection
135692pMB22.SV40.ZeoLentivirus delivery for stable expression of AsCas12a-VPR, Zeocin selection
135694psiCHECK2-miR-200b-3pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-200b)
135695psiCHECK2-miR-26b-5pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-26b)
135696psiCHECK2-miR-876Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-876)
135697psiCHECK2-miR-309Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-309)
135698psiCHECK2-miR-182-5pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-182)
135699psiCHECK2-miR-543Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-543)
135700psiCHECK2-miR-653Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-653)
135701psiCHECK2-miR-134Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-134)
135702psiCHECK2-miR-125Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-125)
135703psiCHECK2-miR-137Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-137)
135704psiCHECK2-miR-133Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-133)
135705psiCHECK2-miR-101Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-101)
135706psiCHECK2-miR-497Mammalian expression vector, Luciferase reporter to test activity of miRNA (miR-497)
135707psiCHECK2-miR-193bMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-193)
135708psiCHECK2-miR-16-5pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-16-5p)
135709psiCHECK2-miR-15-5pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-15-5p)
135710psiCHECK2-miR-19-3pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-19-3p)
135711psiCHECK2-miR-23a-3pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-23a-3p)
135712psiCHECK2-miR-96-5pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-96-5p)
135713psiCHECK2-miR-106b-5pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-106-5p)
135714psiCHECK2-miR-130a-3pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-130a-3p)
135716psiCHECK2-miR-142-3pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-142-3p)
135717psiCHECK2-miR-144-3pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-144-3p)
135718psiCHECK2-miR-145-5pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-145-5p)
135719psiCHECK2-miR-183-5pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-183-5p)
135720psiCHECK2-miR-194-5pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-194-5p)
135721psiCHECK2-miR-199a-5pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-199a-5p)
135722psiCHECK2-miR-203a-3pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-203a-3p)
135723psiCHECK2-miR-223-3pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-223-3p)
135724psiCHECK2-miR-489-3pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-489-3p)
135725psiCHECK2-miR-503-5pMammalian expression vector, Luciferase reporter to test activity of miRNA (miR-503-5p)

Antibodies from Article