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Viral manipulation of functionally distinct interneurons in mice, non-human primates and humans.

Vormstein-Schneider D, Lin JD, Pelkey KA, Chittajallu R, Guo B, Arias-Garcia MA, Allaway K, Sakopoulos S, Schneider G, Stevenson O, Vergara J, Sharma J, Zhang Q, Franken TP, Smith J, Ibrahim LA, M Astro KJ, Sabri E, Huang S, Favuzzi E, Burbridge T, Xu Q, Guo L, Vogel I, Sanchez V, Saldi GA, Gorissen BL, Yuan X, Zaghloul KA, Devinsky O, Sabatini BL, Batista-Brito R, Reynolds J, Feng G, Fu Z, McBain CJ, Fishell G, Dimidschstein J
Nat Neurosci. 2020 Dec;23(12):1629-1636. doi: 10.1038/s41593-020-0692-9. Epub 2020 Aug 17. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
135630pAAV-S5E2-dTom-nlsdTomAAV vector to drive the expression of dTomato in PV cortical interneurons under the control of the E2 regulatory element
135631pAAV-S5E2-GFP-fGFPAAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element
135632pAAV-S5E2-GCaMP6fAAV vector to drive the expression of GCaMP6f in PV cortical interneuronsunder the control of the E2 regulatory element
135633pAAV-S5E2-C1V1-eYFPAAV vector to drive the expression of C1V1-eYFP in PV cortical interneuronsunder the control of the E2 regulatory element
135634pAAV-S5E2-ChR2-mCherryAAV vector to drive the expression of ChR2-mCherry in PV cortical interneuronsunder the control of the E2 regulatory element
135635pAAV-S5E2-Gq-P2A-dTomatoAAV vector to drive the expression of Gq-DREADD-P2A-dTomato in PV cortical interneuronsunder the control of the E2 regulatory element
135637pAAV-S5E1-dTom-nlsdTomAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E1 regulatory element
135638pAAV-S5E3-dTom-nlsdTomAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E3 regulatory element
135639pAAV-S5E4-dTom-nlsdTomAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E4 regulatory element
135640pAAV-S5E5-dTom-nlsdTomAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E5 regulatory element
135641pAAV-S5E6-dTom-nlsdTomAAV vector to drive the expression of dTomato in VIP cortical interneurons under the control of the E6 regulatory element
135642pAAV-S5E7-dTom-nls-dTomAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E7 regulatory element
135643pAAV-S5E8-dTom-nlsdTomAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E8 regulatory element
135644pAAV-S5E9-dTom-nls-dTomAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E9 regulatory element
135645pAAV-S5E10-dTom-nlsdTomAAV vector to drive the expression of dTomato in PV cortical interneuronsunder the control of the E10 regulatory element
153434pAAV-E11-ChR2GFP2xAAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E11 regulatory element
153435pAAV-E14-ChR2GFP2xAAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E14 regulatory element
153436pAAV-E22-ChR2GFP2xAAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E22 regulatory element
153437pAAV-E29-ChR2GFP2xAAV vector to drive the expression of dChr2-GFP-P2A-GFP in PV cortical interneuronsunder the control of the E29 regulatory element
219432pAAV_S5E2_Chrimson_tdTomatoAAV construct expressing Chrimson-tdTomato under the control of E2 regulatory element

Antibodies from Article