RABIF/MSS4 is a Rab-stabilizing holdase chaperone required for GLUT4 exocytosis.
Gulbranson DR, Davis EM, Demmitt BA, Ouyang Y, Ye Y, Yu H, Shen J
Proc Natl Acad Sci U S A. 2017 Sep 26;114(39):E8224-E8233. doi: 10.1073/pnas.1712176114. Epub 2017 Sep 11.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135556 | pET-28a-His6-SUMO-RABIF | Express human RABIF in E. coli BL21, purified via HisPur Ni-NTA Resin |
| 135557 | pFastBac HT JS-Rab10wt | Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt. |
| 135558 | pcDNA3.1(+)-RABIF-FLAG | Express human RABIF in mammalian cells, FLAG at N terminal |
| 135559 | pcDNA3.1(+)-Rab10-FLAG | Express mouse Rab10 in mammalian cells, FLAG at N terminal |