Functional Reconstitution of Intracellular Vesicle Fusion Using Purified SNAREs and Sec1/Munc18 (SM) Proteins.
Yu H, Crisman L, Stowell MHB, Shen J
Methods Mol Biol. 2019;1860:237-249. doi: 10.1007/978-1-4939-8760-3_15.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135550 | pET-28a-His6-SUMO-Munc18-1wt | Express rat Munc18-1 in E. coli BL21, purified via HisPur Ni-NTA Resin |
| 135551 | pET-28a-His6-SUMO-VAMP2 | Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin |
| 135552 | pET-28a-His6-SUMO-VAMP3 | Express rat VAMP3 in E. coli BL21, purified via HisPur Ni-NTA Resin |
| 135553 | pET-28a-His6-SUMO-VAMP8 | Express mouse VAMP8 in E. coli BL21, purified via HisPur Ni-NTA Resin |
| 135554 | pFastBac HT JS-Munc18b | Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b. |
| 135555 | pFastBac HT JS-Munc18c | Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c. |