The structure of human SFPQ reveals a coiled-coil mediated polymer essential for functional aggregation in gene regulation.
Lee M, Sadowska A, Bekere I, Ho D, Gully BS, Lu Y, Iyer KS, Trewhella J, Fox AH, Bond CS
Nucleic Acids Res. 2015 Apr 20;43(7):3826-40. doi: 10.1093/nar/gkv156. Epub 2015 Mar 12.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135436 | pCDF11-SFPQ(276-535) | Expresses SFPQ 276-535 with TEV-cleavable hexahistidine tag |
| 135437 | pCDF11-SFPQ(276-598) | Expresses SFPQ 276-598 with TEV-cleavable hexahistidine tag |
| 135438 | pCDF11-SFPQ(369-598) | Expresses SFPQ 369-598 with TEV-cleavable hexahistidine tag |
| 135439 | pCDF11-SFPQ(214-535) | Expresses SFPQ 214-535 with TEV-cleavable hexahistidine tag |
| 135440 | pCDF11-SFPQ(214-598) | Expresses SFPQ 214-598 with TEV-cleavable hexahistidine tag |
| 135441 | pCDF11-SFPQ(214-598)_quadruple_mutant | Expresses SFPQ 214-598 quadruple mutant (L535A, L539A, L546A, M549A) with TEV-cleavable hexahistidine tag |
| 135442 | pET-Duet-1-NONO(53-312) | Expresses NONO 53-312 in the second site; for co-expression with His-tagged protein |