An improved auxin-inducible degron system preserves native protein levels and enables rapid and specific protein depletion.
Sathyan KM, McKenna BD, Anderson WD, Duarte FM, Core L, Guertin MJ
Genes Dev. 2019 Oct 1;33(19-20):1441-1455. doi: 10.1101/gad.328237.119. Epub 2019 Aug 29.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 126580 | pMGS46 (ARF16-PB1-HA-P2A-OsTIR1) | A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus |
| 126581 | pMGS36 (GFP-ARF16-PB1) | Construct to express GFP-ARF16-PB1 under the control of the CMV promoter |
| 126582 | pMGS7 (AAVS1sgRNA) | A CRISPR/Cas9 construct to target the AAVS1 locus in humans |
| 126583 | pMGS54 (AID-P2A-Hygromycin) | An AID-P2A-HygR cassette to tag the protein of interests with auxin-inducible degron at the c-terminal |
| 129667 | pMGS55 (GFP-ARF16-PB1-MCS-P2A-OsTIR1) | A repair construct to express GFP-ARF16-PB1-MCS and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus |
| 129668 | pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1) | A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus |
| 135311 | pMGS58 | A HygR-P2A-AID cassette to tag the protein of interest with auxin-inducible degron at the n-terminal |